Coding
Modified C

Part:BBa_K4488024:Design

Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li   Group: iGEM22_Sydney_Australia   (2022-10-01)


Modified cellulose binding domain from Clostridium thermocellum cellulosomal scaffold protein A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cytosine at position 153 was changed to thymine and thymine at position 256 was changed to guanine. No restriction sites needed to be removed.


Source

The sequence was adapted from the 2014 Imperial team (BBa_K1321014).

References